一步法人乳铁传递蛋白(LTF)酶联免疫吸附检测试剂盒

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Product Name:一步法人乳铁传递蛋白(LTF)酶联免疫吸附检测试剂盒
Alternative Names:HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
Species:Human
Assay Type:Sandwich
Sensitivity:2.85 ng/mL
standard:200 ng/mL
Detection range:3.13-200 ng/mL
Sample type:Serum, plasma
Assay length:1.5h
Research Area:Tumor immunity;Infection immunity;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LTF, and the Human LTF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human LTF. After TMB substrate solution is added, only those wells that contain Human LTF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.

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