一步法人胰岛素样生长因子结合蛋白7(IGFBP7)酶联免疫吸附检测试剂盒
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Product Name:一步法人胰岛素样生长因子结合蛋白7(IGFBP7)酶联免疫吸附检测试剂盒
Alternative Names:FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor
Species:Human
Assay Type:Sandwich
Sensitivity:1.4 ng/mL
standard:200 ng/mL
Detection range:3.13-200 ng/mL
Sample type:Serum, plasma
Assay length:1.5h
Research Area:Metabolic pathway;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP7, and the Human IGFBP7 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP7. After TMB substrate solution is added, only those wells that contain Human IGFBP7 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.
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