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> 一步法人血管内皮生长因子165(VEGF165)酶联免疫吸附检测试剂盒
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一步法人血管内皮生长因子165(VEGF165)酶联免疫吸附检测试剂盒  

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Product Name：一步法人血管内皮生长因子165(VEGF165)酶联免疫吸附检测试剂盒
Alternative Names：VEGF165
Species：Human
Assay Type：Sandwich
Sensitivity：15 pg/mL
standard：2000 pg/mL
Detection range：31.25-2000 pg/mL
Sample type：Serum, plasma
Assay length：1.5h
Research Area：Cytokine;Tumor immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165, and the Human VEGF165 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGF165. After TMB substrate solution is added, only those wells that contain Human VEGF165 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGF165 in the samples is then determined by comparing the OD of the samples to the standard curve.

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