一步法人膜联蛋白A1(ANXA1)酶联免疫吸附检测试剂盒

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Product Name:一步法人膜联蛋白A1(ANXA1)酶联免疫吸附检测试剂盒
Alternative Names:ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein
Species:Human
Assay Type:Sandwich
Sensitivity:16.8 pg/mL
standard:20 ng/mL
Detection range:0.32-20 ng/mL
Sample type:Serum, plasma
Assay length:1.5h
Research Area:Tumor immunity;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANXA1, and the Human ANXA1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human ANXA1. After TMB substrate solution is added, only those wells that contain Human ANXA1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANXA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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