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一步法人雌二醇(E2)酶联免疫吸附检测试剂盒

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Product Name：一步法人雌二醇(E2)酶联免疫吸附检测试剂盒
Alternative Names：17B-Estradiol; Oestradiol; Beta-Estradiol
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：25 pg/mL
standard：1600 pg/mL
Detection range：25-1600 pg/mL
Sample type：Serum
Assay length：1.5h
Research Area：Endocrinology;Reproductive science;Hormone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human E2 antigen, and the Human E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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