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> 一步法人铁蛋白(FE)酶联免疫吸附检测试剂盒
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一步法人铁蛋白(FE)酶联免疫吸附检测试剂盒

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Product Name：一步法人铁蛋白(FE)酶联免疫吸附检测试剂盒
Alternative Names：Ferritin
Species：Human
Assay Type：Sandwich
Sensitivity：3.2 pg/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Serum
Assay length：1.5h
Research Area：Metabolic pathway;Tumor immunity;Hematology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE, and the Human FE standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human FE. After TMB substrate solution is added, only those wells that contain Human FE and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.

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