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氯喹 二磷酸盐  分析标准品

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熔点：200 °C
包装规格：250mg in glass bottle
溶解性：溶于水
储备液保存：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
体内实验：1、请依序添加每种溶剂： PBS Solubility: 100 mg/mL (193.85 mM); 澄清溶液; 超声助溶 (<60°C) ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
靶点：Plasmodium；Malaria；TLRs；SARS-COV-2；HIV-1；
体外研究：Chloroquine (CHQ，20 μM) 抑制 IL-12p70 释放并降低活化的人单核细胞衍生的朗格汉斯样细胞 (MoLC) 的 Th1 启动能力。Chloroquine (CHQ，20 μM) 增强 MoLC 中 IL-1 诱导的 IL-23 分泌，随后增加致敏 CD4+ T 细胞的 IL-17A 释放。 Chloroquine (25 μM) 在亲本 MDA-MB-231 细胞中抑制常氧和缺氧条件下的 MMP-9 mRNA 表达。Chloroquine phosphate 对 MMP-2、MMP-9 和 MMP-13 mRNA 表达具有细胞、剂量和缺氧依赖性作用。 使用 IRS-954 或 Chloroquine phosphate 抑制 TLR7 和 TLR9 在体外显著降低 HuH7 细胞增殖。 Chloroquine phosphate (0.01-100 μM；48 小时) 有效阻断病毒感染 (vero E6 细胞) 感染 SARS-CoV-2 的低微摩尔浓度 (EC50=1.13 μM)。Chloroquine phosphate 通过增加病毒/细胞融合所需的内体 pH 值以及干扰 SARS-CoV细胞受体的糖基化来阻断病毒感染。
体内研究：Chloroquine (80 mg/kg，ip) 不会阻止原位小鼠模型中具有高或低 TLR9 表达水平的三阴性 MDA-MB-231 细胞的生长。 使用 IRS-954 或 Chloroquine phosphate 抑制 TLR7 和 TLR9 可显著抑制小鼠异种移植模型中的肿瘤生长。DEN/NMOR 大鼠模型中的 HCC 发展也被 Chloroquine phosphate 显著抑制。
细胞实验：The cells are cultured in 6-well plates with normal culture medium in the presence of vehicle or 25 or 50 μM chloroquine, until near confluency, after which they are rinsed with sterile phosphate-buffered saline (PBS) and cultured further for the indicated times in serum-free culture medium. At the desired time-points, the culture medium is discarded and the cells are quickly harvested in lysis buffer and clarified by centrifugation. Subsequent to boiling the supernatants in reducing sodium dodecyl sulfate (SDS) sample buffer, equal amounts of protein (100 μg) are loaded per lane and the samples are electrophoresed into 10 or 4-20% gradient polyacrylamide SDS gels, then transferred to a nitrocellulose membrane. To detect TLR9, the blots were incubated overnight at 4°C with anti-TLR9 antibodies, diluted 1:500 in Tris-buffered saline with 0.1% (v/v) Tween-20 (TBST). Equal loading is confirmed with polyclonal rabbit anti-actin. Secondary detection is performed with horseradish peroxidase-linked secondary antibodies. The protein bands are visualized by chemiluminescence using an ECL kit .
动物实验：Control and TLR9 siRNA MDA-MB-231 cells (5×105 cells in 100 μL) are inoculated into the mammary fat pads of four-week-old, immune-deficient mice (athymic nude/nu Foxn1). Treatments are started seven days after tumor cell inoculation. The mice are treated daily either with intraperitoneal (i.p.) chloroquine (80 mg/kg) or vehicle (PBS). The animals are monitored daily for clinical signs. Tumor measurements are performed twice a week and tumor volume is calculated according to the formula V=(π/6) (d1×d2)3/2, where d1 and d2 are perpendicular tumor diameters. The tumors are allowed to grow for 22 days, at which point the mice are sacrificed and the tumors are dissected for a final measurement. Throughout the experiments, the animals are maintained under controlled pathogen-free environmental conditions (20-21°C, 30-60% relative humidity and a 12-h lighting cycle). The mice are fed with small-animal food pellets and supplied with sterile water ad libitum .
产品描述：抗疟疾化合物
保存条件：室温
注意事项：1、为了您的安全和健康，请穿实验服并戴一次性手套操作。 2、以上信息仅做参考交流之用。

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