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原儿茶酸 文献数量：1  ≥98%(HPLC),分析标准品

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包装规格：20mg in glass bottle
产品描述：基本信息 产品编号： D10117 产品名称： 3,4-Dihydroxybenzoic acid CAS: 99-50-3   储存条件 粉末 室温 四年     分子式： C7H6O4 溶于液体 -80℃ 两年 分子量 154.12 -20℃ 一个月 化学名：  4-Carboxy-1,2-dihydroxybenzene Solubility (25°C)：   体外:   DMSO 30mg/mL (194.65mM) Ethanol   Water   体内(现配现用): 1.请依序添加每种溶剂：PBS Solubility: 16.67mg/mL (108.16mM); Clear solution; Need ultrasonic 2.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (16.22mM); Clear solution 此⽅案可获得 ≥ 2.5mg/mL (16.22mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 3.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5mg/mL (16.22mM); Clear solution 此⽅案可获得 ≥ 2.5mg/mL (16.22mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。 4.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (16.22mM); Clear solution 此⽅案可获得 ≥ 2.5mg/mL (16.22mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 6.4885mL 32.4423mL 64.8845mL 5mM 1.2977mL 6.4885mL 12.9769mL 10mM 0.6488mL 3.2442mL 6.4885mL 50mM 0.1298mL 0.6488mL 1.2977mL   生物活性 产品描述 Protocatechuic acid 是⼀种天然多酚类物质，具有神经保护的作⽤。 靶点 Microbial Metabolite 体外研究 Protocatechuic acid inhibits the aggregation of Aβ and αS and destabilizes their preformed fibrils. Protocatechuic acid prevents the death of PC12 cells triggered by Aβ- and αS-induced toxicity 体内研究 Protocatechuic acid is able to prevent stress induced immobility time in forced swim test without altering locomotor activity in mice. Further, Protocatechuic acid treatment attenuates the elevation of serum corticosterone, lipid peroxidation and restores enzymatic antioxidants in cerebral cortex and hippocampus in ARS mice. Rat administered cadmium and treated with prostigmine and doses of Protocatechuic acid (10−20mg/kg) has significantly reduced BChE activity. Cadmium and either prostigmine or Protocatechuic acid (10−20mg/kg) treated rats shows significant reduction in MDA level   推荐实验方法(仅供参考) Kinase Assay AChE activity investigation is carried out in a reaction mixture containing 50μL of tissue homogenate, 50μL of 5, 5'- dithiobis-(2-nitrobenzoic) acid (DTNB), 1175μL of 0.1 M phosphate-buffered solution, pH 8.0. After incubation for 20 min at 25°C, 25μL of acetylthiocholine iodide solution is added as the substrate. The AChE activity is determined as changes in absorbance reading at 412nm for 3 min at 25°C and using a UV/Visible spectrophotometer.     Cell Assay Dilutions of Protocatechuic acid (2, 5, 10, 20, 50, and 100μM) are prepared from stock solutions, with serum-free culture medium. Equal volumes of each solution are mixed with Aβ1-42 (10μM), then incubated for 24 h on a thermoblock, with continuous agitation, and then exposed to PC12 cells for 24 h to test whether Protocatechuic acid can prevent cell death triggered by Aβ. Cell viability is determined by MTT reduction assay. Cells are treated with 200μL per well of MTT solution (final concentration, 0.5mg/mL in DMEM-Glutamax medium) for 3 h, at 37°C, with 5% CO2. The dark blue formazan crystals that formed are solubilized with 100μL per well of DMSO, for 30 min. Absorbance is measured at 540nm, with a microplate reader. Results are expressed as the percentage of MTT reduction in relation to the absorbance of control cells at 100%.
保存条件：室温

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