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ML-18  ≥98%

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包装规格：1mg 5mg 10mg 25mg 50mg 100mg in glass bottle
溶解性：溶于DMSO（≥100mg/mL ）
产品描述：基本信息 产品编号： M10924 产品名称： ML-18 CAS: 1422269-30-4   储存条件 粉末 -20℃ 四年     分子式： C32H35N5O5 溶于液体 -80℃ 6个月 分子量： 569.65 -20℃ 1个月 化学名：  (2S)-3-(1H-indol-3-yl)-N-[[1-(4-methoxyphenyl)cyclohexyl]methyl]-2-[(4-nitrophenyl)carbamoylamino]propanamide Solubility (25°C)：   体外:   DMSO   Ethanol ' mg/mL Water   体内(现配现用):   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 1.7555mL 8.7773mL 17.5546mL 5mM 0.3511mL 1.7555mL 3.5109mL 10mM 0.1755mL 0.8777mL 1.7555mL   生物活性 产品描述 一种抑制肺癌生长的非肽铃蟾肽受体亚型-3 (BRS-3) 拮抗剂。 靶点 IC50:4.8μM (BRS-3)   体外研究 ML-18 inhibits specific 125I-BA1 (DTyr-Gln-Trp-Ala-Val-βAla-His-Phe-Nle-NH2)BB6-14 binding to NCI-H1299 lung cancer cells stably transfected with BRS-3 with IC50 values of 4.8μM.ML-18 binds with lower affinity to the GRPR and NMBR with IC50 values of 16 and more than 100μM,respectively.ML-18 at 16μM inhibits the ability of 10nM BA1 to elevate cytosolic Ca2+in a reversible manner using lung cancer cells loaded with FURA2-AM.ML-18 at 16μM inhibits the ability of 100nM BA1 to cause tyrosine phosphorylation of the EGFR and ERK in lung cancer cells.It inhibits the proliferation of lung cancer cells.   推荐实验方法(仅供参考) 激酶实验： The cells are incubated in SIT buffer containing 0.25% bovine serum albumin and 250μg/mL bacitracin and 125I-BA1 (100,000 cpm) is added,as well as various concentrations of unlabelled competitor (ML-18). After incubation at 37℃ for 30 min,free 125I-BA1 is removed by washing 3 times in buffer and the cells which contain bound 125I-BA1 is dissolved in 0.2N NaOH and counted in a gamma counter.The IC50 is calculated for each unlabeled competitor.   细胞实验:   Cell viability is measured using the MTT assay.NCI-H727 or NCI-H1299 cells transfected with BRS-3 are treated with ML-18 (0,4.8,16,48μM) or gefitinib added.After 2 days,15μL of 0.1 % MTT solution added.After 4h,150μL of DMSO is added.After 16h,the optical density at 570nm is determined.
保存条件：-20℃

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