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骨化三醇 促销  ≥99%（HPLC)

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外观与性状：白色或类白色粉末
包装规格：5mg 25mg 100mg in glass bottle
溶解性：溶于乙醇（83mg/ml）、二甲基亚砜（83MG/ML)、甲醇和氯仿
产品描述：基本信息 产品编号： D60002 产品名称： 1α,25-Dihydroxyvitamin D3 CAS: 32222-06-3   储存条件 粉末 -20℃ 四年     分子式： C27H44O3 溶于液体     分子量 416.64     化学名：  1A,25-dihydroxycholecalciferol Solubility (25°C)：   体外:   DMSO 83 mg/mL (199.21mM) Ethanol 83 mg/mL (199.21mM) Water Insoluble 体内(现配现用): 1.请依序添加每种溶剂： 10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: 2.75 mg/mL (6.60mM); Suspended solution; Need ultrasonic 此方案可获得 2.75 mg/mL (6.60mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。 以 1mL 工作液为例，取 100μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加入50μL Tween-80，混合均匀；然后继续加入 450μL生理盐水定容至 1mL。 2.请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline) Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution 此方案可获得 ≥ 2.75 mg/mL (6.60mM，饱和度未知) 的澄清溶液。 以 1mL 工作液为例，取 100μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 3.请依序添加每种溶剂： 10% DMSO→90% corn oil Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution 此方案可获得 ≥ 2.75 mg/mL (6.60mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1mL 工作液为例，取 100μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900μL玉米油中，混合均匀。 4.请依序添加每种溶剂： 5% DMSO→40% PEG300→5% Tween-80→50% saline Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution 5.请依序添加每种溶剂： 5% DMSO→95% (20% SBE-β-CD in saline) Solubility: ≥ 2.75 mg/mL (6.60mM); Clear solution 6.请依序添加每种溶剂： 10% EtOH→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5 mg/mL (6.00 mM); Clear solution 此方案可获得 ≥ 2.5 mg/mL (6.00mM，饱和度未知) 的澄清溶液。 以 1mL 工作液为例，取 100μL 25.0 mg/mL 的澄清 EtOH 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加入50μL Tween-80，混合均匀；然后继续加入 450μL生理盐水定容至 1mL。 7.请依序添加每种溶剂： 10% EtOH→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5 mg/mL (6.00mM); Clear solution 此方案可获得 ≥ 2.5 mg/mL (6.00mM，饱和度未知) 的澄清溶液。 以 1mL 工作液为例，取 100μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 8.请依序添加每种溶剂： 10% EtOH→90% corn oil Solubility: ≥ 2.5 mg/mL (6.00mM); Clear solution 此方案可获得 ≥ 2.5 mg/mL (6.00mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1mL 工作液为例，取 100μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900μL玉米油中，混合均匀。 9.请依序添加每种溶剂： 1% DMSO→99% saline Solubility: 0.55 mg/mL (1.32mM); Suspended solution; Need ultrasonic ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg  1mM 2.4002mL 12.0008mL 24.0015mL 5mM 0.4800mL 2.4002mL 4.8003mL 10mM 0.2400mL 1.2001mL 2.4002mL 50mM 0.0480mL 0.2400mL 0.4800mL   生物活性 产品描述 一种PKC和VDR的激活剂。 靶点 vitamin D receptor (Cell-free assay) 体外研究 Calcitriol exerts antiproliferative effects on cervical cancer cells in vitro. Cells decrease by 12.8% when treated with 100 nM Calcitriol for 6 days, compare with control. Inhibition of cell proliferation becomes more pronounced with the increase in Calcitriol concentration. The decrease is 26.1% and 31.6% for 200 and 500nM Calcitriol, respectively. Treatment with Calcitriol for 72 h induces an evident accumulation of cells in the G1 phase, with approximately 66.18% in 200nM and 78.10% in 500nM, compare with the control (24.36%). Calcitriol treatment significantly decreases HCCR-1 protein expression compare with the control in a time- and dose-dependent manner. Calcitriol significantly increases ERα mRNA in a dose dependent manner with an EC50 of 9.8×10-9 M. 体内研究 Chronic treatment with Calcitriol (150 ng/kg per day for 4.5 months) improves the relaxations (pD2: 6.30±0.09, Emax: 68.6±3.9% in Calcitriol-treated OVX, n=8). Renal blood flow in OVX rats is reduced in both kidneys, and the flow is restored by Calcitriol treatment. The increased expression of COX-2 and Thromboxane-prostanoid (TP) receptor in OVX rat renal arteries is reduced by chronic calcitriol administration. High- and low-dose Calcitriol treatment significantly decreases the systolic blood pressure (SBP) in the fructose-fed rats by 14±4 and 9±4mmHg, respectively, at Day 56. High-dose Calcitriol treatment (20ng/kg per day) significantly increases serum ionized calcium level (1.44±0.05 mmol/L) compare with the other groups. 特征     推荐实验方法(仅供参考) Cell Assay HeLa S3 cells are plated at a density of 1,000 cells/well in 96-well plates of Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), treated with 1% ethanol (control) or various concentrations of Calcitriol (100, 200, and 500nM) for 72 h. A Cell Counting Kit8 (CCK-8) is used to determine cell proliferation. At 24, 48, 72, 96, 120, and 144 h after culturing with 200nM Calcitriol, cells are harvested for analysis. Three independent experiments are performed in quadruplicate   Animal Administration Adult female Sprague-Dawley rats weighing 200 to 220g are used in this study. Rats are housed in a temperature-controlled room (~23°C) with a 12-h light/dark cycle. The animals have free access to a standard diet and water. Ovariectomy (OVX) is performed on rats. At 6 months after the surgical procedure, the OVX rats are randomly assigned to either treatment with vehicle dimethyl sulfoxide (OVX+vehicle) or Calcitriol (150ng/kg daily, OVX+calcitriol). Calcitriol treatment is given by oral gavage and lasted or 4.5 months. Blood pressure and serum Calcitriol level are measured.
保存条件：-20℃

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