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> 马核因子κB亚基p105(NFKB-p105)酶联免疫吸附检测试剂盒
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马核因子κB亚基p105(NFKB-p105)酶联免疫吸附检测试剂盒

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Product Name：马核因子κB亚基p105(NFKB-p105)酶联免疫吸附检测试剂盒
Alternative Names：NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Species：Horse
Assay Type：Sandwich
Sensitivity：0.059 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse NFKB-p105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse NFKB-p105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse NFKB-p105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse NFKB-p105 in the samples is then determined by comparing the OD of the samples to the standard curve.

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