山羊免疫球蛋白M(IgM)酶联免疫吸附检测试剂盒
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Product Name：山羊免疫球蛋白M(IgM)酶联免疫吸附检测试剂盒
Alternative Names：IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region
Species：Goat
Assay Type：Sandwich
Sensitivity：14.3 ng/mL
standard：2000 ng/mL
Detection range：31.25-2000 ng/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Infection immunity;Immune molecule;Hematology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region
Species：Goat
Assay Type：Sandwich
Sensitivity：14.3 ng/mL
standard：2000 ng/mL
Detection range：31.25-2000 ng/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Infection immunity;Immune molecule;Hematology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
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