牛皮肤桥蛋白(DPT)酶联免疫吸附检测试剂盒
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Product Name：牛皮肤桥蛋白(DPT)酶联免疫吸附检测试剂盒
Alternative Names：TRAMP; Tyrosine-rich acidic matrix protein
Species：Cattle
Assay Type：Sandwich
Sensitivity：0.069 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Infection immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle DPT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle DPT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle DPT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle DPT in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：TRAMP; Tyrosine-rich acidic matrix protein
Species：Cattle
Assay Type：Sandwich
Sensitivity：0.069 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Infection immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle DPT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle DPT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle DPT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle DPT in the samples is then determined by comparing the OD of the samples to the standard curve.
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