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> 人转化受体电位阳离子通道亚家族M成员4(TRPM4)酶联免疫吸附检测试剂盒
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人转化受体电位阳离子通道亚家族M成员4(TRPM4)酶联免疫吸附检测试剂盒

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Product Name：人转化受体电位阳离子通道亚家族M成员4(TRPM4)酶联免疫吸附检测试剂盒
Alternative Names：TRPM4B; Melastatin-4; Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4
Species：Human
Assay Type：Sandwich
Sensitivity：0.048 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRPM4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRPM4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRPM4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRPM4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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