人适配器相关蛋白复合物1亚基mu-1(AP1M1)酶联免疫吸附检测试剂盒
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Alternative Names:AP47; CLAPM2; CLTNM; MU-1A; mu1A; Adaptor Related Protein Complex 1 Subunit Mu 1; AP-mu chain family member mu1A
Species:Human
Assay Type:Competitive Inhibition
Sensitivity:0.11 ng/mL
standard:20 ng/mL
Detection range:0.32-20 ng/mL
Sample type:serum, plasma and other biological fluids
Assay length:2h
Research Area:Metabolism;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP1M1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP1M1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP1M1 in the samples is then determined by comparing the OD of the samples to the standard curve.
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