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绵羊胰高血糖素(GC)酶联免疫吸附检测试剂盒

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Product Name：绵羊胰高血糖素(GC)酶联免疫吸附检测试剂盒
Alternative Names：GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
Species：Sheep
Assay Type：Competitive Inhibition
Sensitivity：12.7 pg/mL
standard：2000 pg/mL
Detection range：31.25-2000 pg/mL
Sample type：serum, plasma and other biological fluids
Assay length：2h
Research Area：Endocrinology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep GC in the samples is then determined by comparing the OD of the samples to the standard curve.

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