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硝基酪氨酸(NT)酶联免疫吸附检测试剂盒  

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Product Name：硝基酪氨酸(NT)酶联免疫吸附检测试剂盒
Alternative Names：Nitrotyrosine
Species：General
Assay Type：Competitive Inhibition
Sensitivity：2.1 ng/mL
standard：500 ng/mL
Detection range：7.82-500 ng/mL
Sample type：serum, plasma and other biological fluids
Assay length：2h
Research Area：Metabolic pathway;Infection immunity;Rheumatology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NT in the samples is then determined by comparing the OD of the samples to the standard curve.

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