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Hesperin  ≥98%,A solution in ethanol

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包装规格：5mg 10mg 50mg 100mg in glass bottle
溶解性：溶于乙醇(100mg/mL超声）
产品描述：基本信息 产品编号： H10758 产品名称： Hesperin CAS: 4430-35-7   储存条件 粉末 -20℃ 四年 分子式： C8H15NOs2 溶于液体 -80℃(避光，储于氮) 6个月 分子量： 205.34 -20℃(避光，储于氮) 1个月 化学名：  6-(methylsulfinyl)hexyl isothiocyanate Solubility (25°C)：   体外:   DMSO   Ethanol   Water   体内(现配现用):   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 4.8700mL 24.3499mL 48.6997mL 5mM 0.9740mL 4.8700mL 9.7399mL 10mM 0.4870mL 2.4350mL 4.8700mL   生物活性 产品描述 一种Nrf2活化剂。 靶点 Nrf2   体外研究 Hesperin (6-Methylsulfinylhexyl isothiocyanate,6-MSITC) is an active compound in wasabi (Wasabia japonica Matsum.).Whether Hesperin induces cytotoxicity of HUVECs is determined.More than 1μg/mL of Hesperin markedly induces cytotoxicity and morphological alterations. In subsequent experiments we used Hesperin is used at concentrations of 0-1μg/mL,to study the anti-coagulant and anti-inflammatory properties of Hesperin in HUVECs. 体内研究 Hesperin (6-Methylsulfinylhexyl isothiocyanate,6-MSITC) activates Nrf2 and induces phase II enzyme genes but this induction is absent in Nrf2-null mice,suggesting that Hesperin is a potential activator of the Nrf2/ARE-dependent detoxification pathway.To determine whether Hesperin ameliorates hepatic steatosis and iron accumulation,wild-type and Nrf2-null mice are fed the following diets for 12 weeks:1) control diet,2) high-fat diet (HFD),3) HFD plus Hesperin (10mg/kg/day ip),4) HFD for 6 weeks followed by an iron-supplemented HFD for 6 weeks (HFD/Iron),5) HFD/Iron plus Hesperin.The HFD increased hepatic triglycerides in both genotypes and Hesperin suppress increased hepatic triglycerides in wildtype mice but do not reduce thesetriglycerides in Nrf2-null mice.   推荐实验方法(仅供参考) 细胞实验:   Primary human umbilical vein endothelial cells (HUVECs) are cultured in collagen-coated tissue-culture dishes in an atmosphere containing 95% air and 5% CO2.Human monoblast U937 cells are grown in RPMI-1640 medium with 10% fetal bovine serum,10 U/mL Penicillin,and 10μg/mL Streptomycin.HUVECs are cultured in collagen-coated 96-well plates as confluent monolayers.Hesperin is added into wells at the indicated final concentrations (0-30μg/mL) and then incubated for 24h.Cell viability is measured by cell counting kits.   动物实验:   Mice A colony of wild-type and Nrf2-null mice are backcrossed with C57BL/6 mice for ten generations.All mice are housed in the same animal care facility controlled for temperature,humidity,and light. Seven-week-old male wild-typeand Nrf2- null mice (n=6-8/group) are divided into five groups fed the following diets:1) a standard diet (AIN-93,containing 4% soybean oil) for 12 weeks and vehicle (1:10 solution of DMSO/PBS) injected intraperitoneally 4 times per week for the last four weeks (control group),2) a high-fat diet (HFD) (containing 4% soybean oil and 31% lard) for 12 weeks and vehicle injected intraperitoneally 4 times per week for the last four weeks (HFD group),3) a HFD for 12 weeks and Hesperin (10mg/Kg/day;dissolved in 1:10 solution of DMSO/PBS) injected intraperitoneally 4 times per week for the last four weeks (HFD+ Hesperin),4) a HFD for 6 weeks followed by a HFD containing 1% carbonyl iron for 6 weeks and vehicle injected intraperitoneally 4 times per week for the last four weeks(HFD+Iron),and 5) a HFD for 6 weeks followed by a HFD containing 1% carbonyl iron for 6 weeks and Hesperin (10mg/Kg/day) injected intraperitoneally 4 times per week for the last four weeks (HFD+Iron+Hesperin).After 12 weeks,blood samples are collected by cardiac puncture under anesthesia with sodium pentobarbital (50mg/kg,ip) and livers are harvested and stored at -80℃ until analysis.
保存条件：-20℃

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