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RG14620  ≥98%

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包装规格：5mg 10mg 25mg 50mg 100mg in glass bottle
溶解性：溶于DMSO（30mg/ml超声）
产品描述：基本信息 产品编号：R10442 产品名称：RG14620 CAS: 136831-49-7   储存条件 粉末 -20℃ 四年 分子式： C14H8Cl2N2 溶于液体 -80℃ 两年 分子量 275.13     化学名：      Solubility (25°C)   体外 DMSO 55 mg/mL (199.9 mM) Ethanol 6 mg/mL (21.8 mM) Water Insoluble 体内 （现配现用）   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 3.6346mL 18.1732mL 36.3465mL 5mM 0.7269mL 3.6346mL 7.2693mL 10mM 0.3635mL 1.8173mL 3.6346mL 50mM 0.0727mL 0.3635mL 0.7269mL   生物活性 产品描述 一种EGFR抑制剂；IC50值为3μM。 靶点/IC50 EGFR  ABCG2   体外研究 RG14620 inhibits colony formation (IC50=3μM) and DNA synthesis (IC50=1μM) by HER 14 cells, which are stimulated by 50ng/mL EGF, in a dose-dependent manner. RG14620 also suppresses colony formation(IC50=4μM) and DNA synthesis (IC50 =1.25μM) by EGF-stimulated MH-85 cells in a dose-dependent manner. The growth-inhibitory effect of RG14620 irreversible   体内研究 RG14620, at a dose of 200g/mouse/day inhibits H-85 tumor growth in nude mice. Mice show less cachexia and hypercalcemia, eat more food, and are more active than untreated MH-85 tumor-bearing animals.   推荐实验方法(仅供参考) 细胞实验:   MH-85 cells and HER 14 cells are plated in complete medium, either αMEM or DMEM, respectively, supplemented with 10% FCS. After overnight culture, the culture medium is switched to αMEM supplemented with 0.2% PCS and 50ng/mL EGF (MH85) or DMEM supplemented with 0.5% PCS and 50ng/mL EGF (HER14). The cells are cultured in this medium in the presence or absence of increasing concentrations of RG-13022 or RG-14620 for 10 days. At the end of culture, the cells are fixed with 4% (v/v) formaldehyde in calcium-magnesium-free phosphate-buffered saline for 15min at room temperature and stained with hematoxylin. Numbers of colonies including more than 20 cells in each well are counted under the microscope.   动物实验:   Mice: RG14620 in 0.1mL 100% DMSO is injected i.p. twice a day from 1day after MH-85 tumor inoculation. Control animals are given the same vehicle.
保存条件：-20℃

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