生物试剂
>
生化试剂
>
信号通路
> Didox
加入收藏 取消收藏

Didox  ≥98%

促销价¥{{model.attbuying.price}}

市场价¥0.00

累计销量0 累计评价0 人评论

别称 {{model.alias}}
中文名称 {{model.name}}
英文名称 {{model.enname}}
品牌 {{model.brand}}
CAS {{model.cas}}
分子式
分子量 {{model.molecularweight}}
MDL {{model.mdl}}
货号 {{model.procode}}
数量

+ -

件 库存{{model.attbuying.number}}件
立即购买 加入购物车 下载说明书

看了又看
包装规格：10mg 50mg 100mg in glass bottle
溶解性：溶于DMSO（100 mg/mL 超声）
产品描述：基本信息 产品编号：D10804 产品名称：Didox CAS: 69839-83-4   储存条件 粉末 -20℃ 四年     分子式： C7H7NO4 溶于液体 -80℃ 六个月 分子量 169.13 -20℃ 一个月 化学名：  3,4-Dihydroxybenzohydroxamic acid   Solubility (25°C)   体外 DMSO 100mg/mL (591.26mM; Need ultrasonic) Ethanol   Water   体内 现配现用   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 5.9126mL 29.5631mL 59.1261mL 5mM 1.1825mL 5.9126mL 11.8252mL 10mM 0.5913mL 2.9563mL 5.9126mL   生物活性 产品描述 一种合成的核糖核苷酸还原酶 (ribonucleotide reductase, RR) 抑制剂。 靶点/IC50 Ribonucleotide reductase   体外研究 Didox (NSC-324360) suppresses LPS-induced mRNA levels of iNOS, IL-6, IL-1, TNF-α, NF-κβ (p65), and p38-α, after 24 h of treatment. Treatment with Didox also suppresses the secretion of nitric oxide (NO), IL-6, and IL-10. Using mitochondrial dehydrogenase activity as a measure of cytotoxicity, the effects of Didox on cellular respiration in RAW264.7 are examined over a range of concentrations for 24h. Cells exposures to 200μM and below Didox, with and without LPS, do not exhibit significant cellular toxicity. Didox (NSC-324360) is active against all human and murine acute myeloid leukemia (AML) lines tested with IC50 values in the low micromolar range (mean IC50 37 µM [range 25.89-52.70 µM]).   体内研究 Once engraftment is established by bioluminescent imaging, the animals receive daily administrations of Didox at 425mg/kg via IP injection over 5 days. Didox (NSC-324360) treatment significantly reduces leukemic burden compared to vehicle treated controls (p=0.0026 and p=0.0342). More importantly, Didox (NSC-324360) provides a significant survival benefit(p<0.0001 and p=0.0094).   推荐实验方法(仅供参考) 细胞实验： Cell Assay RAW264.7 macrophages are treated with Didox alone, with 0.1μg/mL LPS, or the two in combination. Cellular respiration, as an indication of cytotoxicity, is measured by the MTT assay, which quantifies mitochondrial dehydrogenase activity. Macrophages are plated into 96 well Costar plates at 105  cells per well in 100μL of DMEM media. After 4h of incubation at 37°C for adherence, compounds and DMSO carrier control (0.01% final) are added in triplicate over serial dilutions beginning with 200μM per well in a total volume of 200μL, and the plates incubated for 24 h. Four h before termination of the assay, each well receives 20μL of a 5 mg/mL MTT solution in un-supplemented DMEM. After centrifugation, the supernatant for each well is discarded and cells containing reduced MTT are solubilized with 100μL of acidified isopropanol(4 mM HCl, 0.1% NP-40 in isopropanol). Following a brief period of shaking, the optical density (O.D.) for each well is recorded at 550nm. Each experiment is repeated three times and the data averaged from each triplicate, then expressed as percentage of the control O.D. values for each experiment   动物实验:   Animal Administration Mice Luciferase-tagged leukemia cells are transplanted into 8- week old, sublethally irradiated (4.5Gy) C57Bl/6 mice by tail vein injection of 1.0×106  cells per mouse. Mice are injected with 150mg/kg D-Luciferin, anesthetised with Isoflurane, and imaged using the IVIS 100 imaging system. Mice begin treatment with Didox upon detection of clear signal. The animals are treated with daily administrations of Didox (NSC-324360) at 425mg/kg Didox by intraperitoneal injection (IP) for 5 days. Control animals receive 5% dextrose water by IP injection. Repeat imaging is performed on the day following the final treatment.
保存条件：-20℃

猜你喜欢