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α-NETA  ≥98%

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包装规格：5mg in glass bottle
溶解性：溶于DMSO(2mg/mL)
产品描述：基本信息 产品编号： N10630 产品名称： α-NETA CAS: 115066-04-1   储存条件 粉末 -20℃ 四年     分子式： C16H20InO 溶于液体 -80℃ 6个月 分子量 369.24 -20℃ 1个月 化学名：  trimethyl-(3-naphthalen-1-yl-3-oxopropyl)azanium;iodide Solubility (25°C)：   体外:   DMSO 80mg/mL (216.66mM; Need ultrasonic) Ethanol   Water   体内(现配现用): 1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80 →45% saline Solubility: ≥ 2.08 mg/mL (5.63mM); Clear solution 此⽅案可获得 ≥ 2.08 mg/mL (5.63mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 2.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.08 mg/mL (5.63mM); Clear solution 此⽅案可获得 ≥ 2.08 mg/mL (5.63mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均 匀。 3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.08 mg/mL (5.63mM); Clear solution 此⽅案可获得 ≥ 2.08 mg/mL (5.63mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 2.7083mL 13.5413mL 27.0827mL 5mM 0.5417mL 2.7083mL 5.4165mL 10mM 0.2708mL 1.3541mL 2.7083mL     生物活性 产品描述 一种有效的，非竞争性的胆碱乙酰转移酶 (ChA) 抑制剂，IC50 为 9μM。 靶点 IC50: 9μM (ChAT);    0.04 µM (ALDH1A1); CMKLR1; 84 µM (ChE); 300 µM (AChE)Hedgehog     体外研究 α-NETA (50-150nM; 24 hours) decreases all cell lines viability in a dose-dependent manner. α-NETA (2.5-10.0 µg/mL; 24 hours) leads to epithelial ovarian cancer (EOC) cell death associated with membrane blistering and cytoplasm leakage. α-NETA treatment increases EOC cell expression of pyroptosis-associated proteins. α-NETA is most potent in inhibiting aldehyde dehydrogenase 1 family, member A1 (ALDH1A1; IC50=0.04 µM; purified enzymes assay), followed by CMKLR1 (IC50=0.375 µM for β-ARR2 recruitment; Cell-based assay) and G9a histone lysine methyltransferase (IC50=0.50 µM; purified enzymes assay). α-NETA selectively inhibits chemerin-stimulated CMKLR1 association with β-arrestin2. α-NETA possesses fluorescent characteristics (excitation spectrum: maxima 255 and 297nm; emission spectrum: maximum 437nm) of naphthyl moiety.   Cell Viability Assay Cell Line: Ho8910, Ho8910PM, A2780, and Iose80 cells Concentration: 50, 100, 150nM Incubation Time: 24 hours Result: Decreased all cell lines viability in a dose-dependent manner. Apoptosis Analysis Cell Line: Epithelial ovarian cancer (EOC) cell Concentration: 2.5, 7.5, 10.0 µg/mL Incubation Time: 24 hours Result: Led to EOC cell death associated with membrane blistering and cytoplasm leakage. 体内研究 α-NETA (i.p.; 0.125 mg/kg; once every other day for 20 days) significantly decreases tumor volume and tumor weight. α-NETA (s.c. injection; 3 mg/kg or 10 mg/kg; daily; for 30 days) significantly delays the onset of EAE with 3 mg/kg, and completely suppresses clinical signs for an average of nine days with 10 mg/kg beyond the first appearance of disease in control female C57BL/6 mice. Animal Model: BALB/c nude mice with skov3 cells Dosage: 0.125 mg/kg Administration: IP; once every other day for 20 days Result: Significantly decreased tumor volume and tumor weight.
保存条件：-20℃

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