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RG 13022  ≥98%

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产品描述：基本信息 产品编号：R10128 产品名称：RG 13022 CAS: 136831-48-6   储存条件 粉末 -20℃ 四年     分子式： C16H14N2O2 溶于液体 -80℃ 六个月 分子量 266.30 -20℃ 一个月 化学名：      Solubility (25°C)   体外 DMSO 53mg/mL (199.03mM) Ethanol 5mg/mL (18.77mM)   Water Insoluble 体内（现配现用）     ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 3.7553mL 18.7765mL 37.5530mL 5mM 0.7511mL 3.7553mL 7.5106mL 10mM 0.3755mL 1.8777mL 3.7553mL 50mM 0.0751mL 0.3755mL 0.7511mL   生物活性 产品描述 一种EGF抑制剂,在HT-22细胞中IC50值为1μM。 靶点/IC50 EGFR(Cell-free assay) 4μM   体外研究 RG13022 suppresses EGF-stimulated cancer cell proliferation. In a cell-free reaction RG13022 inhibits the autophosphorylation reaction of the EGF receptor in immunoprecipitates with an IC50 of 4μM. RG13022 inhibits colony formation and DNA synthesis by HER 14 cells, which were stimulated by 50 ng/mL EGF, in a dose-dependent manner. The IC 50s are 1 and 3μM for HER 14 colony formation and DNA synthesis, respectively. RG-13022 inhibits not only EGF-induced growth but also growth stimulated by insulin, insulin-like growth factor I, insulin-like growth factor II, or transforming growth factor alpha. RG-13022 also totally blocks estrogen-stimulated phosphorylation of the EGF receptor, as well as estrogen-induced cell proliferation, suggesting that functioning TK pathways are required for estrogen action.   体内研究 RG13022 suppresses tumor growth in nude mice. RG13022 also increases the life span of these tumor-bearing nude mice.   推荐实验方法(仅供参考) 细胞实验:   RG13022 suppresses EGF-stimulated cancer cell proliferation. In a cell-free reaction RG13022 inhibits the autophosphorylation reaction of the EGF receptor in immunoprecipitates with an IC50 of 4μM. RG13022 inhibits colony formation and DNA synthesis by HER 14 cells, which were stimulated by 50ng/mL EGF, in a dose-dependent manner. The IC 50s are 1 and 3μM for HER 14 colony formation and DNA synthesis, respectively. RG-13022 inhibits not only EGF-induced growth but also growth stimulated by insulin, insulin-like growth factor I, insulin-like growth factor II, or transforming growth factor alpha. RG-13022 also totally blocks estrogen-stimulated phosphorylation of the EGF receptor, as well as estrogen-induced cell proliferation, suggesting that functioning TK pathways are required for estrogen action.   动物实验:   Mice: MH-85 tumors 5mm in diameter are inoculated s.c. into the right dorsal portion of 4- to 6-week-old male BALB/c nu/nu mice. RG-13022 or RG-14620 in 0.1ml 100% DMSO is injected i.p. twice a day from 1day after MH-85 tumor inoculation.Control animals are given the same vehicle. Tumor sizes are measured once a week under anesthesia with nembutal (0.05mg/g body weight, i.p.) and calculated.
保存条件：-20℃

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