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甲基牛扁亭柠檬酸盐  ≥98%

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包装规格：5mg in glass bottle
溶解性：溶于DMS（250mg/ml 超声 加热）(水:1.67mg/ml超声)
产品描述：基本信息 产品编号： M11282 产品名称： Methyllycaconitine citrate CAS: 351344-10-0   储存条件 粉末 -20℃ 四年     分子式： C43H58N2O17 溶于液体 -80℃ 6个月 分子量： 874.92 -20℃ 1个月 化学名：  MLA Solubility (25°C)：   体外:   DMSO   Ethanol   Water   体内(现配现用):   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 1.1430mL 5.7148mL 11.4296mL 5mM 0.2286mL 1.1430mL 2.2859mL 10mM 0.1143mL 0.5715mL 1.1430mL   生物活性 产品描述 α7神经元烟碱型乙酰胆碱受体(α7nAChR)的特异性拮抗剂。 靶点 α7nAChR   体外研究 Pretreatment with 5 and 10µM Methyllycaconitine citrate (MLA) inhibits the decreased cell viability induced by Aβ25-35.Cell viability does not decrease after exposure to Methyllycaconitine citrate (2.5,5,10,20µM).Aβ25-35 treatment increases LC3-II levels,which is inhibited by administration of Methyllycaconitine citrate.Methyllycaconitine citrate also inhibits Aβ-induced autophagosome accumulation in SH-SY5Y cells.Flow cytometry also demonstrates decreased MDC-labeled vacuoles with Methyllycaconitine citrate treatment. 体内研究 Methyllycaconitine citrate (MLA) (6mg/kg) given alone intraperitoneally does not cause climbing behavior when compare with the saline group.Pretreatment with Methyllycaconitine citrate significantly inhibits methamphetamine (METH)-induced climbing behavior,by about 50%.Methyllycaconitine citrate does not modify either basal locomotor activity or METH-induced hyperlocomotion.The METH-induced depletion of dopamine neuron terminals is attenuated in mice pretreated with Methyllycaconitine citrate (250±43 fmol/mg,n=7).A direct effect of Methyllycaconitine citrate on body temperature is ruled out because Methyllycaconitine citrate does not affect basal body temperature (37.0±0.5℃,n=5) or reduce the METH-induced hyperthermia (38.2±0.4℃,n=6,MLA+METH group,n.s.versus METH group).   推荐实验方法(仅供参考) 细胞实验:   Cells are plated in 96-well plates containing complete medium and cultured for 24h.Then cells are treated with Methyllycaconitine citrate at the indicated concentrations for specified times.After drug treatment,cell viability is measured by MTT assay.Briefly,10µL of the MTT solution (5mg/mL) is added to each well and incubated for 4 h at 37℃.After removing the supernatant,100µL DMSO is added into each well.The absorbance is measured at 570nm with a microplate reader.All experiments are repeated 3 times.   动物实验:   Adult male Swiss CD-1 mice are used in all experiments.They are housed at 22±1℃ under a 12-h light/dark cycle with free access to food and drinking water.Climbing behavior is measured.Briefly,mice of 20 to 26g are intraperitoneally administered saline (5mL/kg) or Methyllycaconitine citrate (MLA) (6mL/kg) at the beginning of the test.Twenty minutes later,the animals receive a single dose of saline or methamphetamine (METH) (1mL/kg) subcutaneously and are placed individually,for habituation,into the experimental chamber consisting of a cylindrical cage with the wall made of plastic bars and covered with a lid.After a 20-min period of exploratory activity,stereotypy measurement is performed for a period of 30 min.
保存条件：-20℃

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