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PF-573228  ≥98%

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包装规格：10mg 50mg 100mg 500mg 1g 5g in glass bottle
溶解性：溶于DMSO（≥ 51 mg/mL）
产品描述：基本信息 产品编号：P10798 产品名称：PF-573228 CAS: 717906-29-1   储存条件 粉末 -20℃ 四年     分子式： C22H20F3N5O3S 溶于液体 -80℃ 六个月 分子量 491.49 -20℃ 一个月 化学名：      Solubility (25°C)   体外 DMSO   Ethanol   Water   体内（现配现用）     ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 2.0346mL 10.1731mL 20.3463mL 5mM 0.4069mL 2.0346mL 4.0693mL 10mM 0.2035mL 1.0173mL 2.0346mL   生物活性 产品描述 是有效的和选择性的FAK抑制剂，对FAK纯化重组催化片段的IC50值为4nM。 靶点/IC50 IC50: 4nM (FAK   体外研究 PF-573228 inhibits purified recombinant catalytic fragment of FAK with an IC50 of 4nM. In cultured cells, PF-573228 inhibits FAK phosphorylation on Tyr397 with an IC50 of 30-100nM.Treatment of cells with concentrations of PF-573228 that significantly decreased FAK Tyr397 phosphorylation fails to inhibit cell growth or induce apoptosis. In contrast, treatment with PF-573228 inhibits both chemotactic and haptotactic migration concomitant with the inhibition of focal adhesion turnover. 特征 Imatinib 是多靶点酪氨酸激酶抑制剂。      推荐实验方法(仅供参考) 激酶实验： Purified activated FAK kinase domain is reacted with 50μM ATP, and 10μg/well of a random peptide polymer of Glu and Tyr (molar ratio of 4:1), poly (Glu/Tyr) in kinase buffer for 15min.Phosphorylation of poly(Glu/Tyr) is challenged with s. 细胞实验:   REF52 or PC3 cells are seeded into a 24-well plate in triplicate 24h prior to daily treatment with the indicated concentrations of each inhibitor (PF-573228) for 3 days. Subsequently, the cells are harvested and counted. Apoptosis assays are performed using a cell death detection ELISA.REF52,PC3 or MDCKcells are treated for 24h (16h for MDCK) with the indicated concentrations of each inhibitor prior to lysis. Cells suspended for 16-24h in serum-free medium served as a positive control. The cell lysates are incubated in duplicate in the ELISA system. The data represent the means±standard deviation of one of three experiments performed in duplicate.
保存条件：-20℃

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