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三乙烯硫代磷酰胺  ≥98%

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包装规格：50mg 100mg 250mg 1g in glass bottle
溶解性：溶于DMSO（≥ 50 mg/mL)
产品描述：基本信息 产品编号：T10556 产品名称：Thio-TEPA CAS: 52-24-4   储存条件 粉末 2-8℃ 四年 分子式： C6H12N3PS 溶于液体 -80℃ 两年 分子量 189.22 -20℃ 一个月 化学名：  Phosphorothioic triamide, N,N',N''-tri-1,2-ethanediyl-   Solubility (25°C)   体外 DMSO 100mg/mL (528.48mM) Ethanol 100mg/mL (528.48mM) Water 100mg/mL warmed with 50ºC water bath (528.48mM) 体内 现配现用   ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 5.2849mL 26.4243mL 52.8485mL 5mM 1.0570mL 5.2849mL 10.5697mL 10mM 0.5285mL 2.6424mL 5.2849mL 50mM 0.1057mL 0.5285mL 1.0570mL   生物活性 产品描述 一种 DNA 烷化剂，具有抗肿瘤作用。 靶点/IC50 DNA Alkylator   体外研究 Thio-TEPA exhibits alkylating activity in rat liver slice incubation. Thio-TEPA does not affect the viability of rat liver slices,and is not accumulated in the slices at all doses of 2, 5, 10mM.   体内研究 Thio-TEPA (20mg/kg, i.p.) with total body irradiation (TBI) enhances donor-type blood chimerism during the first 10 weeks but is not dramatically higher than that of TBI group alone. Thio-TEPA alone improves both short- and long-term engraftment in mice. 特征     推荐实验方法(仅供参考) 细胞实验:   Cell Assay The effect of Thio-TEPA on slice viability is studied by the determination of intracellular potassium (K+) leakage. Intracellular K+ is measured by flame photometry and is expressed in relation to the DNA content as measured using fluorometry. ThioTEPA is added at concentrations of 1, 2, 5, and 10mM and slices are incubated for 6, 12, and 24 h. The results are compared with the values found for drug-free controls. Thio-TEPA in medium and slices is measured following 3, 6, and 9 h incubation at the three highest concentrations. Krebs-HEPES buffer is used as the slicing buffer and Waymouth's MB 752/1 medium supplemented with 10μg gentamycin is used as the incubation medium.   动物实验:   Animal Administration Mice Male C57BL/6JIco mice, 19 weeks old, 25 to 30 g, are used as recipients. Congenic C57BL/6J-Gpi-1a/Gpi-1a (B6-Gpi-1a) and BALB.B10LiLa (Gpi-1a) mice are used as a source of syngeneic and allogeneic donor bone marrow, respectively. The latter combination is H-2-compatible (both H-2b), but mismatched on a number of minor histocompatibility loci. Thio-TEPA is dissolved in PBS and injected i.p. at a single bolus of 20mg/kg. Preliminary pilot experiments have established that this approximated to the maximal tolerated dose when higher doses result in lethal gastrointestinal toxicity. Cyclophosphamide (CY) is dissolved in PBS and administered i.p. at a single dose of 200mg/kg. Total body irradiation is delivered using a 137Cs γ-irradiation unit at a dose rate of 87cGy/min to a dose of 5Gy. After Thio-TEPA treatment the mice are maintained on neomycin (3.5g/L sterile drinking water) for 2weeks to minimize the possible problems of endotoxemia via gut toxicity.
保存条件：2-8℃

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