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> 人双特异性酪氨酸磷酸化调节激酶1A(DYRK1A)酶联免疫吸附检测试剂盒
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人双特异性酪氨酸磷酸化调节激酶1A(DYRK1A)酶联免疫吸附检测试剂盒  

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Product Name：人双特异性酪氨酸磷酸化调节激酶1A(DYRK1A)酶联免疫吸附检测试剂盒
Alternative Names：DYRK; DYRK1; HP86; MNB; MNBH; Dual specificity YAK1-related kinase; Protein kinase minibrain homolog
Species：Human
Assay Type：Sandwich
Sensitivity：0.118 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Enzyme & Kinase;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DYRK1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DYRK1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DYRK1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DYRK1A in the samples is then determined by comparing the OD of the samples to the standard curve.

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