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> 人腺苷A2a受体(ADORA2a)酶联免疫吸附检测试剂盒
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人腺苷A2a受体(ADORA2a)酶联免疫吸附检测试剂盒  

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Product Name：人腺苷A2a受体(ADORA2a)酶联免疫吸附检测试剂盒
Alternative Names：ADORA2; RDC8; hA2AR
Species：Human
Assay Type：Sandwich
Sensitivity：0.06 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：CD & Adhesion molecule;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADORA2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADORA2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADORA2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADORA2a in the samples is then determined by comparing the OD of the samples to the standard curve.

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