生物试剂
>
生化试剂
>
信号通路
> IPA-3
加入收藏 取消收藏

IPA-3  ≥98%

促销价¥{{model.attbuying.price}}

市场价¥0.00

累计销量0 累计评价0 人评论

别称 {{model.alias}}
中文名称 {{model.name}}
英文名称 {{model.enname}}
品牌 {{model.brand}}
CAS {{model.cas}}
分子式
分子量 {{model.molecularweight}}
MDL {{model.mdl}}
货号 {{model.procode}}
数量

+ -

件 库存{{model.attbuying.number}}件
立即购买 加入购物车 下载说明书

看了又看
包装规格：1mg 5mg 10mg 50mg 100mg in glass bottle
溶解性：溶于DMSO（≥100mg/mL）
产品描述：基本信息 产品编号：I10746 产品名称：IPA-3 CAS: 42521-82-4   储存条件 粉末 -20℃ 四年     分子式： C20H14O2S2 溶于液体 -80℃ 六个月 分子量 350.45 -20℃ 一个月 化学名：      Solubility (25°C)   体外 DMSO 70mg/mL (199.74mM) Ethanol 70mg/mL (199.74mM) Water Insoluble ＜1mg/ml表示微溶或不溶。 普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。   制备储备液   浓度   溶液体积 质量   1mg   5mg   10mg 1mM 2.8535mL 14.2674mL 28.5347mL 5mM 0.5707mL 2.8535mL 5.7069mL 10mM 0.2853mL 1.4267mL 2.8535mL 50mM 0.0571mL 0.2853mL 0.5707mL   生物活性 产品描述 一种选择性的，ATP 非竞争的 PAK1 抑制剂，IC50 值为 2.5μM，对 PAKs 4-6 没有抑制作用。 靶点/IC50 PAK1(Cell-free assay) 2.5μM 体外研究 IPA-3 inhibits Pak1 activation in part by binding covalently to the regulatory domain of Pak1. IPA-3 binds Pak1 covalently in a time- and temperature-dependent manner. IPA-3 prevents binding of the Pak1 activator Cdc42. IPA-3 binds directly to the Pak1 autoregulatory domain. IPA-3 reversibly inhibits PMA-induced membrane ruffling in cells. IPA-3 (2µM, 5µM or 20µM) reduces cell spreading in human primary Schwann and schwannoma cells. IPA-3 treatment significantly reduces the number of adherent Schwann and schwannoma cells in a dose-dependent manner. IPA-3 is a non ATP-competitive, allosteric inhibitor of p21-activated kinase 1 (Pak1).PIR3.5 is the control compound of IPA-3.IPA-3 prevents Cdc42-stimulated Pak1 autophosphorylation on Thr423. IPA-3 also prevents sphingosine-dependent Pak1 autophosphorylation. IPA-3 does not target exposed cysteine residues on Pak1. The disulfide bond of IPA-3 is critical for inhibition of Pak1 and in vitro reduction by the reducing agent dithiothreitol (DTT) abolishes Pak1 inhibition by IPA-3. IPA-3 inhibits activation of Pak1 by diverse activators, but does not inhibit preactivated Pak1. IPA-3 inhibits PDGF-stimulated Pak activation in mouse embryonic fibroblasts.IPA-3 inhibits Pak1 activation in part by binding covalently to the regulatory domain of Pak1. IPA-3 binds Pak1 covalently in a time- and temperature-dependent manner. IPA-3 prevents binding of the Pak1 activator Cdc42. IPA-3 binds directly to the Pak1 autoregulatory domain. IPA-3 reversibly inhibits PMA-induced membrane ruffling in cells. IPA-3 (2µM, 5µM or 20µM) reduces cell spreading in human primary Schwann and schwannoma cells. IPA-3 treatment significantly reduces the number of adherent Schwann and schwannoma cells in a dose-dependent manner. IPA-3 is a non ATP-competitive, allosteric inhibitor of p21-activated kinase 1 (Pak1).PIR3.5 is the control compound of IPA-3.IPA-3 prevents Cdc42-stimulated Pak1 autophosphorylation on Thr423. IPA-3 also prevents sphingosine-dependent Pak1 autophosphorylation. IPA-3 does not target exposed cysteine residues on Pak1. The disulfide bond of IPA-3 is critical for inhibition of Pak1 and in vitro reduction by the reducing agent dithiothreitol (DTT) abolishes Pak1 inhibition by IPA-3. IPA-3 inhibits activation of Pak1 by diverse activators, but does not inhibit preactivated Pak1. IPA-3 inhibits PDGF-stimulated Pak activation in mouse embryonic fibroblasts.   推荐实验方法(仅供参考) 激酶实验： Pak1 (150nM final) is pre-incubated with MBP (8.3μM), indicated proteins, and IPA-3 or DMSO in Kinase buffer for 20 minutes at 4℃. Cdc42-GTPγS (3.2μM) is then added and the reaction is pre-equilibrated 10 minutes at 30℃. Kinase reactions are started by the addition of ATP (to 30μM) containing [32P]ATP and are incubated 10 min and analyzed by SDSPAGE and autoradiography.   细胞实验:   Human primary schwannoma cells are grown on 96 well plates for 2 days. Cells are left untreated or treated with 5µM IPA-3,20µM IPA-3 or 20µM PIR-3.5 for 24 hours. The MTS-solution is left on the cells for 3 hours, before the absorbance at 490 nm is measured. The experiments are conducted three times and mean and standard error of the mean is calculated with Excel.
保存条件：-20℃

猜你喜欢