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> 猪90kDa热休克蛋白β1(HSP90b1)酶联免疫吸附检测试剂盒
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猪90kDa热休克蛋白β1(HSP90b1)酶联免疫吸附检测试剂盒

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Product Name：猪90kDa热休克蛋白β1(HSP90b1)酶联免疫吸附检测试剂盒
Alternative Names：HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1; 94 kDa glucose-regulated protein; Endoplasmin
Species：Pig
Assay Type：Sandwich
Sensitivity：0.31 ng/mL
standard：50 ng/mL
Detection range：0.79-50 ng/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;Immune molecule;Developmental science;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HSP90b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HSP90b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HSP90b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HSP90b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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