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> 兔孕酮(PG)酶联免疫吸附检测试剂盒
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兔孕酮(PG)酶联免疫吸附检测试剂盒

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Product Name：兔孕酮(PG)酶联免疫吸附检测试剂盒
Alternative Names：P4; Pregn-4-Ene-3,20-Dione
Species：Rabbit
Assay Type：Competitive Inhibition
Sensitivity：0.47 ng/mL
standard：100 ng/mL
Detection range：1.57-100 ng/mL
Sample type：serum, plasma and other biological fluids
Assay length：2h
Research Area：Endocrinology;Reproductive science;Hormone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit PG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PG in the samples is then determined by comparing the OD of the samples to the standard curve.

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