人游离前列腺特异性抗原(F-PSA)酶联免疫吸附检测试剂盒
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Product Name：人游离前列腺特异性抗原(F-PSA)酶联免疫吸附检测试剂盒
Alternative Names：F-PSA
Species：Human
Assay Type：Sandwich
Sensitivity：17.31 pg/mL
standard：2000 pg/mL
Detection range：31.25-2000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Tags & Cell Markers;Neuroscience;Signal Transduction;Cancer
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-PSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-PSA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：F-PSA
Species：Human
Assay Type：Sandwich
Sensitivity：17.31 pg/mL
standard：2000 pg/mL
Detection range：31.25-2000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Tags & Cell Markers;Neuroscience;Signal Transduction;Cancer
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-PSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-PSA in the samples is then determined by comparing the OD of the samples to the standard curve.
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