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> 绵羊生长激素受体(GHR)酶联免疫吸附检测试剂盒
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绵羊生长激素受体(GHR)酶联免疫吸附检测试剂盒

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Product Name：绵羊生长激素受体(GHR)酶联免疫吸附检测试剂盒
Alternative Names：GHBP; Somatotropin receptor; Growth hormone-binding protein; Serum-binding protein
Species：Sheep
Assay Type：Sandwich
Sensitivity：0.057 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;Endocrinology;Hormone metabolism;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep GHR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep GHR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep GHR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep GHR in the samples is then determined by comparing the OD of the samples to the standard curve.

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