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> 人左旋肉碱/L-肉碱酶联免疫吸附检测试剂盒
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人左旋肉碱/L-肉碱酶联免疫吸附检测试剂盒

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Product Name：人左旋肉碱/L-肉碱酶联免疫吸附检测试剂盒
Alternative Names：L-Carnitine
Species：Human
Assay Type：Sandwich
Sensitivity：0.119 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：serum, plasma, urine, tissue homogenates
Assay length：3.5h
Research Area：Biochemicals
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human L-Carnitine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human L-Carnitine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human L-Carnitine in the samples is then determined by comparing the OD of the samples to the standard curve.

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