大鼠犬尿喹啉酸(KYNA)酶联免疫吸附检测试剂盒

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Product Name:大鼠犬尿喹啉酸(KYNA)酶联免疫吸附检测试剂盒
Alternative Names:Kinurenic acid; Kynuronic acid; Kuinurenic acid; Transtorine
Species:Rat
Assay Type:Competitive Inhibition
Sensitivity:9.35 ng/mL
standard:2000 ng/mL
Detection range:31.25-2000 ng/mL
Sample type:serum, plasma and other biological fluids
Assay length:2h
Research Area:Enzyme & Kinase;
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat KYNA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KYNA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KYNA in the samples is then determined by comparing the OD of the samples to the standard curve.

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