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> 人高亲和力cGMP特异性3’,5’-环磷酸二酯酶9A(PDE9A)酶联免疫吸附检测试剂盒
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人高亲和力cGMP特异性3’,5’-环磷酸二酯酶9A(PDE9A)酶联免疫吸附检测试剂盒

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Product Name：人高亲和力cGMP特异性3’,5’-环磷酸二酯酶9A(PDE9A)酶联免疫吸附检测试剂盒
Alternative Names：High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A ; PDE9A
Species：Human
Assay Type：Sandwich
Sensitivity：0.052 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma,Tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDE9A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDE9A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDE9A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDE9A in the samples is then determined by comparing the OD of the samples to the standard curve.

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