小鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫吸附检测试剂盒
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Product Name：小鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫吸附检测试剂盒
Alternative Names：ACP5;TRAP5b;SPENCDI
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.46 ng/mL
standard：50 ng/mL
Detection range：0.78-50 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Immunology;Cardiovascular
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：ACP5;TRAP5b;SPENCDI
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.46 ng/mL
standard：50 ng/mL
Detection range：0.78-50 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Immunology;Cardiovascular
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.
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