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> 人N端中段骨钙素(N-MID-OT)酶联免疫吸附检测试剂盒
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人N端中段骨钙素(N-MID-OT)酶联免疫吸附检测试剂盒

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Product Name：人N端中段骨钙素(N-MID-OT)酶联免疫吸附检测试剂盒
Alternative Names：N-MID-OT
Species：Human
Assay Type：Sandwich
Sensitivity：0.39 ng/mL
standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human N-MID-OT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human N-MID-OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human N-MID-OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human N-MID-OT in the samples is then determined by comparing the OD of the samples to the standard curve.

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