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> 大鼠白细胞分化抗原8(CD8)酶联免疫吸附检测试剂盒
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大鼠白细胞分化抗原8(CD8)酶联免疫吸附检测试剂盒

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Product Name：大鼠白细胞分化抗原8(CD8)酶联免疫吸附检测试剂盒
Alternative Names：p32; CD8-A; Leu2; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; Cluster Of Differentiation 8a
Species：Rat
Assay Type：Sandwich
Sensitivity：0.062 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD8 in the samples is then determined by comparing the OD of the samples to the standard curve.

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