25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒
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Product Name：25-羟基维生素D(25-OH-D)酶联免疫吸附检测试剂盒
Alternative Names：25 Hydroxy Vitamin D;
Species：General
Assay Type：Competitive Inhibition
Sensitivity：0.9 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：serum, plasma and other biological fluids
Assay length：2h
Research Area：Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：25 Hydroxy Vitamin D;
Species：General
Assay Type：Competitive Inhibition
Sensitivity：0.9 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：serum, plasma and other biological fluids
Assay length：2h
Research Area：Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
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