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> 大鼠Kelch样ECH关联蛋白1(KEAP1)酶联免疫吸附检测试剂盒
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大鼠Kelch样ECH关联蛋白1(KEAP1)酶联免疫吸附检测试剂盒

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Product Name：大鼠Kelch样ECH关联蛋白1(KEAP1)酶联免疫吸附检测试剂盒
Alternative Names：INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19
Species：Rat
Assay Type：Sandwich
Sensitivity：0.057 ng/mL
standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Signal transduction;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KEAP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KEAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KEAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KEAP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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