人抗凝固蛋白抗体(anti-CarP)酶联免疫吸附检测试剂盒

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Product Name:人抗凝固蛋白抗体(anti-CarP)酶联免疫吸附检测试剂盒
Alternative Names:anti- CarP
Species:Human
Assay Type:Sandwich
Sensitivity:0.31 ng/mL
standard:50 ng/mL
Detection range:0.79-50 ng/mL
Sample type:Serum, plasma and other biological fluids.
Assay length:3.5h
Research Area:Biochemical research
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-CarP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-CarP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-CarP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human anti-CarP in the samples is then determined by comparing the OD of the samples to the standard curve.

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