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> 大鼠雷帕霉素靶蛋白(mTOR)酶联免疫吸附检测试剂盒
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大鼠雷帕霉素靶蛋白(mTOR)酶联免疫吸附检测试剂盒

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Product Name：大鼠雷帕霉素靶蛋白(mTOR)酶联免疫吸附检测试剂盒
Alternative Names：FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target
Species：Rat
Assay Type：Sandwich
Sensitivity：0.125 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
Assay length：3.5h
Research Area：Cell proliferation
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MTOR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MTOR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MTOR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MTOR in the samples is then determined by comparing the OD of the samples to the standard curve.

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