鸡卵黄免疫球蛋白(IgY)酶联免疫吸附检测试剂盒
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Product Name：鸡卵黄免疫球蛋白(IgY)酶联免疫吸附检测试剂盒
Alternative Names：Immunoglobulin Yolk
Species：Chicken
Assay Type：Competitive Inhibition
Sensitivity：1.81 µg/mL
standard：400 µg/mL
Detection range：6.25-400 µg/mL
Sample type：serum, plasma and other biological fluids
Assay length：2h
Research Area：Infection immunity;Immune molecule;Hematology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken IgY protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IgY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IgY in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：Immunoglobulin Yolk
Species：Chicken
Assay Type：Competitive Inhibition
Sensitivity：1.81 µg/mL
standard：400 µg/mL
Detection range：6.25-400 µg/mL
Sample type：serum, plasma and other biological fluids
Assay length：2h
Research Area：Infection immunity;Immune molecule;Hematology;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken IgY protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IgY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IgY in the samples is then determined by comparing the OD of the samples to the standard curve.
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