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> 小鼠免疫球蛋白G3(IgG3)酶联免疫吸附检测试剂盒
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小鼠免疫球蛋白G3(IgG3)酶联免疫吸附检测试剂盒

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Product Name：小鼠免疫球蛋白G3(IgG3)酶联免疫吸附检测试剂盒
Alternative Names：IGHG3; HDC; Heavy chain disease protein; Ig Gamma-3 Chain C Region; Immunoglobulin Heavy Constant Gamma 3; G3m Marker; Immunoglobulin Gm3
Species：Mouse
Assay Type：Sandwich
Sensitivity：1.41 µg/mL
standard：200 µg/mL
Detection range：3.13-200 µg/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length：3.5h
Research Area：Infection immunity;Immune molecule;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IgG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgG3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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