人γ-氨基丁酸B受体关联蛋白(gABARBP)酶联免疫吸附检测试剂盒

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Product Name:人γ-氨基丁酸B受体关联蛋白(gABARBP)酶联免疫吸附检测试剂盒
Alternative Names:GABARBP
Species:Human
Assay Type:Sandwich
Sensitivity:0.065 ng/mL
standard:10 ng/mL
Detection range:0.16-10 ng/mL
Sample type:Tissue homogenates, cell lysates and other biological fluids.
Assay length:3.5h
Research Area:Signal transduction;
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GABARBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GABARBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GABARBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GABARBP in the samples is then determined by comparing the OD of the samples to the standard curve.

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