Product Name：牛诱导型一氧化氮合成酶(NOS2/iNOS)酶联免疫吸附检测试剂盒
Alternative Names：NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
Species：Cattle
Assay Type：Sandwich
Sensitivity：0.124 ng/mL
standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids.
Assay length：3.5h
Research Area：Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.

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