植物吲哚乙酸(IAA)酶联免疫吸附检测试剂盒
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Product Name：植物吲哚乙酸(IAA)酶联免疫吸附检测试剂盒
Alternative Names：IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
Species：Plant
Assay Type：Competitive Inhibition
Sensitivity：0.88 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：plant tissues
Assay length：2h
Research Area：Signal transduction;Hormone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant IAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant IAA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative Names：IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
Species：Plant
Assay Type：Competitive Inhibition
Sensitivity：0.88 ng/mL
standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：plant tissues
Assay length：2h
Research Area：Signal transduction;Hormone metabolism;
Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant IAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant IAA in the samples is then determined by comparing the OD of the samples to the standard curve.
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