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> 大鼠白介素1β前体(pro-IL1b)酶联免疫吸附检测试剂盒
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大鼠白介素1β前体(pro-IL1b)酶联免疫吸附检测试剂盒

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Product Name：大鼠白介素1β前体(pro-IL1b)酶联免疫吸附检测试剂盒
Alternative Names：pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β
Species：Rat
Assay Type：Sandwich
Sensitivity：17.2 pg/mL
standard：4000 pg/mL
Detection range：62.5-4000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length：3.5h
Research Area：Cytokine;Tumor immunity;Infection immunity;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

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