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绵羊抵抗素(RETN)酶联免疫吸附检测试剂盒

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Product Name：绵羊抵抗素(RETN)酶联免疫吸附检测试剂盒
Alternative Names：ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3
Species：Sheep
Assay Type：Sandwich
Sensitivity：12 pg/mL
standard：2000 pg/mL
Detection range：31.25-2000 pg/mL
Sample type：serum, plasma and other biological fluids
Assay length：3.5h
Research Area：Metabolic pathway;Endocrinology;Cardiovascular biology;
Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep RETN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep RETN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep RETN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep RETN in the samples is then determined by comparing the OD of the samples to the standard curve.

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