白藜芦醇(RVT)酶联免疫吸附检测试剂盒

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Product Name:白藜芦醇(RVT)酶联免疫吸附检测试剂盒
Alternative Names:trans-3,5,4'-Trihydroxystilbene; 3,4',5-Stilbenetriol; trans-Resveratrol
Species:General
Assay Type:Competitive Inhibition
Sensitivity:91.1 ng/mL
standard:20000 ng/mL
Detection range:312.5-20000 ng/mL
Sample type:plant tissue and biological fluids
Assay length:2h
Research Area:Cardiocerebral vascular system
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with RVT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RVT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RVT in the samples is then determined by comparing the OD of the samples to the standard curve.

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